Fig. 2

Characterization of expanded UCB-CD34⁺ cells in the presence/absence of different combinations of small molecules. TNC fold expansion, CD34⁺ cells percentage, fold expansion of CD34⁺ cells, and colony-forming potential of UCB-CD34⁺ cells was evaluated in each experiment. a 7 SM cocktail (SB, PD, Chir, Bpv, NAM, Pur, Pμ) and its derivative groups b 6 SMs cocktail (SB, Chir, Bpv, NAM, Pur, Pμ) and its derivative groups c 4 SMs cocktail (SB, Chir, Bpv, Pμ) and its derivative groups d 3 SMs cocktail (SB, Chir, Bpv). CD34⁺ cells cultivated in presence of SCF, FLT3L, and TPO were used as a positive control. Fold expansion was determined by dividing the total number of viable cells expressing the phenotype at the end of the culture by the input number of viable cells expressing the same phenotype (n = 3). A statistically significant difference compared with a positive control group, *P ≤ 0.05, **P ≤ 0.01,***P ≤ 0.001