Fig. 3

Establishment of mouse distal airway injury model. a Schematic showing experimental design. Corn oil or naphthalene was injected intraperitoneally into mice at 0 days. At 1 days, DMEM and differentiated iPSCs were intratracheally administered in the control and iPS groups, respectively. Mice were sacrificed at 5 days (n = 4 for all groups) and 15 days (n = 4 for NA group and iPS group, n = 5 for control group). b Preparation of naphthalene-treatment model. Corn oil and NA groups are shown from the second day after treatment. In the NA group, CCSP-positive club cells (green) were completely detached. NA, naphthalene treatment; iPSCs, induced pluripotent stem cells; IT, intratracheal injection; BW, body weight; H&E, haematoxylin and eosin; CCSP, club cell secretory protein; DAPI, 4′,6-diamidino-2-phenylindole dihydrochloride