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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: TUG1 enhances high glucose-impaired endothelial progenitor cell function via miR-29c-3p/PDGF-BB/Wnt signaling

Fig. 5

Involvement of the autophagy pathway in the TUG1-mediated effects on HG-treated EPCs. a Western blot analysis of LC3 levels after TUG1 overexpression or chloroquine (CQ) (10 μM) treatment in EPCs. b Relative quantification of LC3 protein levels after treatment with TUG1 overexpression or CQ on EPCs. c Immunofluorescence of LC3 protein (red) after treatment with TUG1 overexpression or CQ on EPCs. Scale bar = 50 μm. d Fluorescence intensity of LC3 protein (red) after treatment with TUG1 overexpression or CQ on EPCs. *p < 0.05, **p < 0.01 vs vector; ##p < 0.01 vs TUG1

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