Fig. 6

TUG1 regulates PDGF-BB-Wnt pathway via miR-29c-3p. a Real-time quantitative PCR confirmed the downregulation of miR-29c-3p in TUG1-overexpressing EPCs. b The predicted positions of miR-29c-3p binding sites in the PDGF-BB and TUG1 transcripts. c Correlation analyses indicated a negative linear association between the expression of TUG1 and miR-29c-3p. d Dual-luciferase reporter gene assay verifies the regulatory relationship between TUG1 and miR-29c-3p, PDGF-BB, and miR-29c-3p. e Western blot analysis of Wnt pathway and PDGF-BB in EPCs transfected with miR-29c-3p mimic and TUG1 overexpression lentivirus. f Relative quantification of protein levels of Wnt pathway and PDGF-BB in EPCs transfected with miR-29c-3p mimic and TUG1 overexpression lentivirus