Fig. 5

Conditioned medium of HNF4α-UMSCs prevented hepatocytes from D-gal-induced injury. a Analysis of viability of mouse primary hepatocytes in different groups at different time points by CCK-8. b Analysis of LDH and AST levels in different groups. c The relative concentrations of proteins of the condition mediums of UMSCs and HNF4α-UMSCs that obtained a significant score (q-value < 0.001%) are presented in a “heatmap”. Low concentration is shown in green, medium concentration is shown in black and high concentration is shown in red. d GO enrichment analysis of biological processes of high-level proteins in CM of HNF4α-UMSCs. Normalized array data of proteins were analyzed by SAM to detect differences in the concentrations between HNF4α-UMSCs-CM and UMSCs-CM. e The levels of LDH and AST in the medium of hepatocytes injured by D-gal with CM of HNF4α-UMSCs-CM, and neutralizing antibodies against different proteins. f ELISA analysis on HB-EGF concentration in the CM of UMSC and HNF4α-UMSC. Data are expressed as means ± S.E.M. (n = 6). *P < 0.05 and **P < 0.01 compared with CON, ^#P < 0.05 and ^##P < 0.01 compared with UMSC-HEP