Fig. 1

Identification of SCAPs and expression of circSIPA1L1 and miR-204-5p in SCAPs during osteoblast differentiation. a Morphology of primary SCAPs. b Relative gene expression of circSIPA1L1, miR-204-5p, and osteoblastic markers of ALPL, RUNX2, and OSX were detected by qRT-PCR at days 0, 3, and 7, respectively. c Tri-lineage differentiation of SCAPs was performed in vitro. a Alizarin red S staining of cells cultured for 14 days in osteogenic induction medium. b Oil red O staining of cells cultured for 28 days in adipogenic induction medium. c Alcian blue staining of cells cultured for 25 days in chondrogenic induction medium. (Scale bar = 200 μm.) d Flow cytometry demonstrated that SCAPs presented high expressions of CD29, CD90, and CD73, but low expressed CD34 and CD45. *P < 0.05, **P < 0.01, and ***P < 0.001