Fig. 2

Phenotypic and functional analysis of ASC-educated M0 Mphs. Flow cytometry analysis of a phagocytosis levels of Zymosan A, E. coli, or S. aureus particles labeled with pHrodo™. Average ± SEM of positive percentages and Geo Mean statistics are shown below; b surface expression of several phagocytic receptors; c surface expression of co-stimulatory molecules; d surface chemokine receptors, in M0 Mphs, in the presence or absence of ASCs; and e Average ± SEM of positive percentages and Geo Mean of the different surface markers. f OLINK analysis of the secretome of M0 Mphs alone, and M0 Mph and ASC co-cultures. Data are representative of at least three independent experiments. The table shows the fold change of NPX between M0 Mphs with ASCs and M0 Mphs alone, together with the p value for this calculation; green indicates the upregulation of targets, and red indicates the downregulation of targets; only statistically significant changes are shown (n = 4). *p < 0.05, **p < 0.01. ASC, adipose-derived mesenchymal stem cell; CCL, C-C motif chemokine; CCR, C-C motif chemokine receptor; CD, cluster of differentiation; CDCP, CUB domain-containing protein; CX3CR, CX3C chemokine receptor; CXCL, C-C-C motif chemokine; CXCR, C-X-C chemokine receptor; E. coli, Escherichia coli; GDNF, glial cell line-derived neurotrophic factor; HLA, human leukocyte antigen; IL, interleukin; IL10RB, IL-10 receptor subunit beta; LIF, leukemia inhibitory factor; MCP, monocyte chemotactic protein; MIP, macrophage inflammatory protein; Mph, macrophage; NGF, nerve growth factor; NPX, normalized protein expression; NT-3, neurotrophin-3; OSM, oncostatin-M; S. aureus, Staphylococcus aureus; TNFRSF, tumor necrosis factor receptor superfamily member; TNFSF, tumor necrosis factor ligand superfamily member