Fig. 1
Cell morphology and adhesion alterations. a Immunofluorescence of OCT4 expression and localization in hHFMSCs, adherent hHFMSCsOCT4, and floating hHFMSCsOCT4. Adherent, adherent hHFMSCsOCT4; Floating, floating hHFMSCsOCT4; NC, negative control. Red represents OCT4, blue for DAPI and green for EGFP. b The morphology of hHFMSCs was gradually altered after the transduction of OCT4. Yellow arrows indicate round floating hHFMSCsOCT4. The first four upper pictures were taken to observe the morphology of the cells under an optical microscope, and the last lower four pictures were taken to observe the expression of EGFP after OCT4 transduction under a fluorescence microscope. These pictures were all taken from randomly selected fields. c Flow cytometric plot of three groups of hHFMSCs and geometric mean of the FSC. d Percentage of single cells adhered to the plates after treatment with dispase. The percentage of disassociated individual cells to the total number of cells is negatively correlated with cell-to-cell adhesion. e Percentage of cells adhered to the Matrigel-coated plates after rinsing with PBS three times. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The error bars represent the standard deviations of measurements in three separate sample runs (n = 3)