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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Investigation of de novo mutations in a schizophrenia case-parent trio by induced pluripotent stem cell-based in vitro disease modeling: convergence of schizophrenia- and autism-related cellular phenotypes

Fig. 5

Assessment of mitochondrial function and tolerance to oxidative stress in the trio NPC lines. a Quantification of reactive oxygen species (ROS) by flow cytometry in NPCs using CellROX kit. Diagram shows the average of mean fluorescence signals ± SE of N = 3 independent experiments. b Effect of CoCl2 treatment on NPC cell survival. NPCs were treated with 125 and 250 μM CoCl2 for 24 h. After 48 h of reoxygenation, cell viability was measured by Presto Blue staining to determine the effect of oxidative stress. The diagram shows the mean values ± SE normalized to untreated cells (N = 5 independent experiments, n = 3 technical replicates/experiments). c Examination of mitochondrial function in NPCs. Fixed NPCs were stained using a fluorescent dye (MitoTracker™ Red CMXRos) and visualized with confocal microscopy. Scale bars = 50 μm. d Quantification of MitoTracker staining. Diagram shows the mean fluorescent intensities ± SE of NPC lines normalized to number of nuclei. Data were generated from confocal microscopy staining with ImageJ (N = 3 independent experiments, n = 4–6 pictures/experiments)

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Stem Cell Research & Therapy

ISSN: 1757-6512