Fig. 2

Characterization of ECs differentiated from the day 5 HEPs derived from the MUSIi011-A cells in endothelial growth medium (EGM). a CD34⁺ cells were isolated from the day 5 HEPs using a magnetic column and cultured in EGM for one passage. The purity of purified CD34⁺ cells after isolation was assessed by flow cytometric analysis. These CD34+ cells were 98% positive for KDR and CD31. b Morphology of ECs after culture in EGM for 1 passage (scale bar = 250 μm). c Flow cytometric result of endothelial markers, CD34, CD31, KDR, and CD144 (VE-cadherin). The CD34⁺CD31⁺ cells were gated for analysis of KDR and CD144. d Immunofluorescent staining for expression of VE-cadherin, CD31, ICAM-1, and vWF. Nuclei were counterstained with DAPI (scale bar = 100 μm). e Functional analysis of iPSC-derived ECs was performed using in vitro tube formation of capillary structures as compared to HUVECs (scale bar = 500 μm) and Ac-LDL uptake (scale bar = 100 μm)