Fig. 4

Erythroid differentiation of the day 8 HSPCs derived from the MUSIi011-A cells in the three-stage erythroid culture system. a Schematic diagram of the three-stage erythroid culture system. b Differentiated erythroid cells stained with Leishman reagent on days 4, 6, 8, 11, 15, and 19 of erythroid differentiation and analyzed by light microscopy. Green arrows represent proerythroblasts, blue arrows represent basophilic erythroblasts, orange arrows represent polychromatic erythroblasts, black arrows represent orthochromatic erythroblasts, and red arrows represent reticulocytes. c Flow cytometric analysis of erythroid markers, CD36, CD49a, GPA, and Band 3, at day 19 of erythroid differentiation. d Growth curve of differentiated cells from day 0 to 19. Error bars represent mean ± SEM of three independent experiments. e An image of cell pellet obtained on day 19 of erythroid differentiation. f Expression of erythroid-associated genes at days 6, 8, 11, and 15 by RT-PCR analysis