Fig. 2
From: Differentiation of human induced pluripotent stem cells into erythroid cells
Differentiation of human iPSCs into RBCs. Human iPSCs can be produced from primary human fibroblasts using Oct4/Klf4/Sox2/LIN28. There are three steps for differentiation of iPSCs into RBCs, including the generation of human iPSCs, HSCs, and RBCs. Several growth factors and cytokines, including SCF, EPO, VEGF, IGF-1, ITS (insulin, transferrin, selenium), dexamethasone (glucocorticoid receptor agonist), TPO, FLT3, BMP4, IL-3, IL-6, and EPO have various functions on human iPSCs differentiation to the erythroid lineage