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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Enhanced differentiation of human pluripotent stem cells into pancreatic endocrine cells in 3D culture by inhibition of focal adhesion kinase

Fig. 5

Upregulation of Cx36 in PECs induced under 3D culture. a Immunostaining of INS and Cx36 in hESC-derived PECs on days 18 and 27, PECs induced under 2D culture with PF-228 (2D+PF-228), fetal islets, and adult islets. Scale bar, 50 μm. b qRT-PCR analysis of Cx36 in PECs induced under 2D culture, 3D culture, and 2D culture treated with PF-228 (2D+PF-228). c Western blot showing Cx36 expression levels in PECs induced under 2D culture, 3D culture, and 2D culture treated with PF-228 (2D+PF-228). Data represent mean ± SD (n = 3), **p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512