Fig. 2

Negative correlation for gene expression kinetics between miR-218 and MEF2C or COL10A1 in MSC during chondrogenic differentiation. a Differential expression of miR-218 between MSC (N = 6 donors, black circles) and AC (N = 4 donors, gray squares) was monitored at indicated time points during chondrogenic differentiation by qRT-PCR; vertical lines correspond to median values; **p ≤ 0.01 (Mann-Whitney U). b MSC (N = 4 donors) were subjected to chondrogenic induction, and expression of MEF2C (black-dotted line) and COL10A1 (black line) mRNA and miR-218 (red-dotted line) was measured by qRT-PCR at indicated time points. Mean values ± SD are shown. c Correlation between COL10A1 and miR-218 expression levels in MSC (N = 4 donors) was measured by qRT-PCR. Pearson correlation coefficient with its p value is indicated