Fig. 2

WJ-MSCs show a variable circRNA expression profile when cocultured with damaged ESCs. a The differences in circRNA expression profiles between the two groups by a circRNA microarray analysis are presented in the form of a heat map. N1–3, non-cocultured WJ-MSCs; T1–3, cocultured WJ-MSCs. Each group consisted of three individuals. CircRNAs are represented by single rows and samples by single columns. b Volcano plot of circRNA microarray analysis data. The values on the X-axes and Y-axes represent normalized fold changes and P values, respectively. The color scale indicates relative expression, upregulation (red) and downregulation (green). CircRNAs with fold change > 2 and P ≤ 0.05 were regarded as differentially expressed. c Circos plot shows the most significantly upregulated (red) and downregulated (green) circRNA expression profiles in the entire genome. d Four upregulated circRNAs’ expression level in WJ-MSCs by qRT-PCR. T, cocultured WJ-MSCs; N, non-cocultured WJ-MSCs. Data in panel are shown as means ± standard deviation (n = 3). ^**P < 0.01 compare to non-coculture group. e Sanger sequencing of circ6401 qPCR amplification products. f Genomic location and splicing mode of circ6401