Fig. 2

Multidifferentiation potential of PD-MSCs^PRL-1. a qRT-PCR analysis of osteogenic markers (OC and COL1A1) in undifferentiated (−) and differentiated (+) PD-MSCs^PRL-1. b Von Kossa staining in osteogenic differentiation of PD-MSCs^PRL-1. Scale bars = 50 μm. c qRT-PCR analysis of adipogenic markers (adipsin and PPAR-γ) in undifferentiated and differentiated PD-MSCs^PRL-1. d Oil Red O staining in adipogenic differentiation of PD-MSCs^PRL-1. Scale bars = 50 μm. e RT-PCR analysis of hepatocyte function markers (albumin, tyrosine aminotransferase; TAT, hepatocyte nuclear factor 1 homeobox A; HNF1A, cytochrome P450 2B6; CYP2B6, and CYP3A4) in undifferentiated and differentiated PD-MSCs^PRL-1. f ICG uptake in PD-MSCs^PRL-1 after hepatogenic differentiation. Scale bars = 50 μm. Data from each group are expressed as the mean ± SD. ^*p < 0.05 versus undifferentiated group. COL1A1, collagen type 1 alpha 1; CYP2B6, cytochrome P450 2B6; HNF1A, hepatocyte nuclear factor 1 homeobox A; OC, osteocalcin; PPAR-γ, peroxisome proliferator-activated receptor gamma; TAT, tyrosine aminotransferase