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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human embryonic stem cell-derived extracellular vesicles alleviate retinal degeneration by upregulating Oct4 to promote retinal Müller cell retrodifferentiation via HSP90

Fig. 4

hESEVs boost retrodifferentiation of Müller cells through MVs in vitro. Müller cells were treated with DiI labeled-MVs or EXOs. Subsequently, TEM was utilized for measurement of morphologies of MVs and EXOs (a), NanoS90 for inspection of MV and EXO sizes (b), and western blot for determination of the expressions of MV and EXO specific proteins (c). DiI labeled-MVs or EXOs were observed by immunofluorescence: green indicates labeled-hESEVs and blue indicates DAPI labeled-nuclear DNA of Müller cells (bar = 25 μm) (d). The fluorescence intensities of Vimentin (e) and CHX10 (f) in retinal Müller cells were performed by immunofluorescence at the 48th hours of treatment; hESEVs, human embryonic stem extracellular vesicles; MVs, microvesicles; EXOs, exosomes; TEM, transmission microscope

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Stem Cell Research & Therapy

ISSN: 1757-6512