Fig. 1

MEG3 affects growth of human liver cancer stem cells. A MEG3 was detected by RT-PCR in pCMV6-A-GFP-hLCSCs, pCMV6-A-GFP-MEG3-hLCSCs, pGFP-V-RS-hLCSCs, and pGFP-V-RS-MEG3. β-actin was used as an internal reference gene. B MEG3 was detected by qRT-PCR. β-actin was used as an internal reference gene. C Dot blotting was used to detect the expression of MEG3. D CircMEG3 was detected by back-to-back RT-PCR. β-actin was used as an internal reference gene. E The cell proliferation ability was determined by CCK8 method. F The percentage of S phase cells was measured by BrdU staining. G (a) Photograph of cell colonies. G (b) Cell colony formation rate. H Sphere formation ability was analyzed. I The photographs of dissected transplanted tumors. J Comparison of weight (g) of transplanted tumors in nude mice. K Comparison of time (days) of transplanted tumors in nude mice. L Xenograft tumor tissue sections (4 μm) were subjected to immunohistochemical staining of anti-PCNA. Comparison of PCNA positive rates in transplanted tumors. M Xenograft tumor tissue sections (4 μm) were subjected to immunohistochemical staining of anti-Ki67. Comparison of Ki67 positive rates in transplanted tumors