Fig. 5

Cytoprotective effects of Mst1 inhibition toward H₂O₂-induced apoptosis in mBM-MSCs. Modulated mBM-MSCs were exposed to 200 μM of H₂O₂ for 12 h. a Representative images of TUNEL-positive mBM-MSC staining in the different groups. The content of TUNEL-positive cells was the number of green points in each image. Scale bar = 500 μm. b Cell apoptosis was analyzed by Annexin V-FITC/PI staining, detected by FACS (n = 3), and quantified on the basis of apoptosis rate (d) (n = 3). c Caspase 3 activity was measured by caspase 3 activity assay (n = 3). d Pro caspase 3 expression was analyzed by Western blot analysis. β-actin was used as the loading control. Data are expressed as mean ± SD. **p < 0.01, *p < 0.05. H₂O₂, mBM-MSC exposed to H₂O₂ only; H₂O₂ + Neg, mBM-MSC/Neg followed by exposure to H₂O₂; H₂O₂ + sh-Mst1, mBM-MSC/sh-Mst1 followed by exposure to H₂O₂; H₂O₂ + sh-Mst1 + 3-MA, mBM-MSCs/sh-Mst1 pretreated with 3-MA followed by exposure to H₂O₂