Fig. 2

Single-cell RNA sequencing (sc-RNAseq) data reveal inter-source variation of equine mesenchymal stromal cells (MSCs). a UMAP plot depicting clustering of MSCs isolated from donor-matched adipose tissue (AT), bone marrow (BM), and peripheral blood (PB). b Heatmap showing significant differentially expressed genes (DEGs) (adjusted p-value < 0.05, log₂ fold change > 1 for each tissue source versus all others, percentage of tissue source with detectable expression of gene > 25%) between tissue sources. Top 10 (ranked by log₂ fold change) genes per tissue source are labeled with gene symbol. For visualization purposes, 250 cells (columns) were randomly selected from each tissue source. c Violin plots of the expression levels of the top 5 DEGs (ranked by log₂ fold change) in MSCs isolated from each tissue source. See also Additional files 1 and 2