Fig. 1
Twist-1del/+ calvarial cells exhibit differential expression of histone demethylases, Kdm6a and Kdm6b. a Gene expression levels of Twist-1, Runx2, Alkaline Phosphatase (Alk Phos), Kdm6a, Kdm6b, and Ezh2 in calvarial cells from wild-type (WT) and Twist-1del/+ (Twist) mice, cultured under osteogenic conditions were analyzed with real-time qPCR and normalized to β-actin. Data represent mean ± S.E., *p < 0.05, two-tailed, not-paired, non-parametric student’s t test, n = 3 WT, and n = 3 Twist mice. b Representative images of calvarial sections focusing on open coronal sutures (white box) of 8-day-old WT and Twist mice using an antibody specific to H3K27me3 (brown stain) counterstained with hematoxylin, scale bar = 100 μm. c Quantitative measurement of the percentage of H3K27me3-positive nuclei to total number of nuclei within the white box using ImageJ software. Data represent mean ± S. E, *p < 0.05, two-tailed, not-paired, non-parametric student’s t test, n = 3 WT, and n = 3 Twist mice)