Fig. 4

Functional annotation of co-different genes and differences in MAPK signaling of adipogenic and myogenic precursors. a The significant KEGG pathways (P < 0.05) enriched with co-different genes between adipogenic and myogenic precursors. Red, purple, and green columns represent pathways related to human diseases, cellular process, and extracellular environmental processing, respectively. The number on the right of column represents the number of genes enriched in each pathway. b Cluster of KEGG pathways and co-different genes. G, gene expression level; M, DNA methylation level. c The expression of MAPK signaling key factors (ERK, JNK, and p38) and their phosphorylated forms (p-ERK, p-JNK, and p-p38) was detected by western blotting between adipogenic and myogenic precursors. d Relative expression level in c was calculated. GAPDH was used as the internal control. Data were presented as means ± SEM (n = 3). The statistical significance of difference between two means was calculated using t test, ^**P < 0.01