Fig. 6

CACNA2D2 negatively regulated myogenic differentiation by inhibiting JNK/MAPK signaling pathway. a Immunofluorescent microscopy analysis of the morphological changes and expression of Myosin in myogenic precursors transfected with CACNA2D2-plasmid or empty vector at 48 h of differentiation. Scale bars, 100 μm. b The mRNA expression level of CACNA2D2 and c–f protein level of CACNA2D2, MyoG, JNK/MAPK signaling key factors (JNK and c-Jun), and their phosphorylated forms (p-JNK and p-c-Jun) were measured in myogenic precursors transfected with CACNA2D2-plasmid or empty vector in growth medium (GM, 0 h) or after 12, 24, and 48 h switched into differentiation medium (DM). GAPDH was used as the internal control. Data were presented as means ± SEM (n = 3). The statistical significance of difference between two means was calculated using t test, ^**P < 0.01