Fig. 3

The BCP improved maturation of hiPSC-CMs. a Hot map of RNA sequencing, those selected representative cardiac maturation-related genes showed significant upregulation in the BCP compared with the monolayer cultured hiPSC-CMs. b Quantitative PCR analyses reconfirmed the upregulated expression of representative cardiac-associated genes, including cardiomyocyte structural genes (TNNI3, MYL6, and MYH7), cardiac conduction-related gene (CAMK2B, KCNQ1), cardiac metabolism-related gene (COX6A2, CKM), and cardiomyocyte maturation markers (NPPA, MYOM3, NKX2.5) as normalized to GAPDH expression. c Transmission electron microscope was used to evaluate the inner cell composition and ultrastructure of the BCP and the hiPSC-CM aggregates on the monolayer culture, Scale bar = 500 nm. d The myofibrillar sarcomere length of hiPSC-CMs on the BCP was significantly longer than that of the hiPSC-CMs. All error bars show mean ± SEM of three independent experiments. Student’s t test was used to compare hiPSC-CM and BCP groups. *P < 0.05 compare with hiPSC-CM group