Fig. 7
Inhibition of miR-146a-5p in Hypo-EVs impaired Hypo-EV-mediated lung protection in a mice model of asthma. a miR-146a-5p expression was detected by real-time PCR in EVs isolated from the hypoxic medium of hUCMSCs treated with 100 nM inhibitor-146a-5p or negative control (NC) (n = 3). b Expression of miR-145a-5p in lung tissue after injection of miR146aKD-Hypo-EVs or NC-Hypo-EVs into the asthma mice (n = 6). c Representative photographs of HE- and PAS-stained lung sections from each group (black bar = 200 μm). d The inflammatory infiltration and goblet cell hyperplasia were quantified by HE and PAS scores (n = 6). e Statistical analysis of the total inflammatory cells and eosinophils in the BALF (n = 6). f Statistical analysis of IL-4 and IL-13 levels in the BALF as measured by ELISA (n = 6). g Representative photomicrographs of airway and parenchyma stained with Masson trichrome staining (black bar = 200 μm). h Percentage of collagen fiber content in airway and lung parenchyma (n = 6). i Representative Western blots of TGF-β1, p-Smad2/3, α-SMA, and collogen-1 in the lungs from each group (n = 3), and band intensities were normalized against the corresponding GAPDH and the numbers are presented as fold increase over the NC-Hypo-EV group. j The expression of p-Smad2/3, α-SMA, and collogen-1 in TGF-β1-treated HLF-1 cells were determined by Western blotting (n = 3). Band intensities were normalized against the corresponding GAPDH and the numbers are presented as fold increase over the NC-Hypo-EV group. *P < 0.05, **P < 0.01, ***P < 0.001