Fig. 7

YAP activation and nuclear translocation contributed to radial shockwave-mediated promotion of self-renewal of SCB-SPCs. a The YAP/TAZ expression level exhibited a consistent tendency with CFU-F under the respective doses of r-ESW stimulation: control group, 1A (1 bar, 300 times), 2B (2 bar, 600 times), and 3D (3 bar, 1200 times) (n = 5, with each 3 technical repeats). b Immunofluorescence of YAP localization and semiqualitative evaluation of YAP distribution in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). c Immunofluorescence of Sox2 expression and semiqualitative evaluation in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). d Immunofluorescence of Nanog expression and semiqualitative evaluation in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). e The relative expression levels of self-renewal-associated genes in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). f The general staining and qualitative analysis of CFU-F among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). Scale bars represent 25 μm. *p < 0.05, **p < 0.01, ***p < 0.001