Fig. 6

ANGPTL3 mediates the angiogenic effects of USC-Exo on HUVECs. a, b The mRNA and protein levels of ANGPTL3 were assessed by RT-qPCR and Western blot analysis. c Western blot analysis of ANGPTL3 in HUVECs after USC-Exo treatment. d Western blot analysis of ANGPTL3 in exosomes from ANGPTL3-silenced USC and confirmation of the inhibitory efficiency of shRNA targeting ANGPTL3. e The proliferation of HUVECs treated with PBS, USC-Con shRNA-Exo, and USC-shANGPTL3-Exo. f Quantitative analysis of the proliferation of HUVECs in different treatment groups shown in e. g, i Representative images of tube formation and migration in HUVECs treated with PBS, USC-Con shRNA-Exo, and USC-shANGPTL3-Exo. h, j Quantitative analyses of the total tube length, total branching points, and total loops shown in g and migration area shown in i. k Western blot analysis of the protein expression levels of PI3K/AKT components to assess signaling pathway activation in HUVECs among the different treatment groups. The data are presented as the means ± SD; n = 6 per group. *p < 0.05, **p < 0.01 compared among different treatment groups