Fig. 2

Characteristics of isolated human liver cells and transduction of genes using adenovirus vector. a A representative phase contrast morphological image of liver cells from passage 6. b Doubling time of liver cells from three different donors in culture media. c Differentiation toward the osteogenic (left: Alizarin Red staining) and adipogenic (right: Oil Red O staining) lineages using specific differentiation cocktails for 21 and 14 days in culture, respectively. d Expression of surface antigens and albumin on liver cells analyzed by flow cytometry (n = 4). Liver cells at early (1–2), mid (6–7), and late (12–14) passages were used. e Immunofluorescence staining of albumin in human liver cells at passages 2, 6, and 14. Green, albumin; blue, nucleus; red arrow, albumin-positive cells. Scale bars represent 200 μm