Fig. 7
a Immunofluorescent staining of senescent markers (P19, P53 and P16) of isolated TDSCs from control and Botox tendon showed increased accumulation of P19 and P53 in the Botox group. But P16 was not significantly changed between both groups. b Western blot of protein expression of P19 and P53 from isolated TDSCs were normalised to beta-actin and showed increased translational level of P19 and P53 in the Botox group. Results were quantified by greyscale density (n = 3; mean ± SD; *p < 0.05, **p < 0.005). c Immunofluorescence staining of P19, P53 and P16 on tendon tissue exhibited higher production of P19 and P53 in the Botox group and again not P16. d Quantification of fluorescence density of P19, P53 and P16 in tendon tissue. Blinded analysis was performed for quantification (n = 3; mean ± SD; *p < 0.05, **p < 0.005)