Fig. 3

Cell proliferation showed by EdU staining and quantitative assay in all groups in day 7 after infection. Representative fluorescence microscopy images (A) and cell number (B) of BALB/c MSCs stained by EdU. Representative fluorescence microscopy images (C) and cell number (D) of C57BL/6 MSCs stained by EdU. Same fixed size of area was selected randomly near the center of the original images of 3 experimental replicate wells. Cell number was count automatically with manually correction using ImageJ (“analyze particles” function). a, p value < 0.05 compared to MSCs-V group; b, p value < 0.05 compared to MSCs-IL-4 group. “+(PDGF)”, add 0.5 ng/ml hPDGF-BB recombinant protein in the culture medium while culturing the cells