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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: The therapeutic efficacy of mesenchymal stromal cells on experimental colitis was improved by the IFN-γ and poly(I:C) priming through promoting the expression of indoleamine 2,3-dioxygenase

Fig. 2

The IFN-γ and poly(I:C)-primed MSCs significantly stimulated the ISC proliferation, enterocyte differentiation, and epithelial regeneration. Mice with the DSS-induced colitis were sacrificed on day 9 to harvest colon tissues. Representative data of two independent experiments are shown (normal, n = 3; other groups, n = 4). a IHC images of colon sections for Ki-67 are presented. The Ki-67-positive cells in crypt are counted from the normal mice, the DSS control, the unstimulated MSC-treated, and the primed MSC-treated mice. b Results of quantitative PCR showed that mRNA levels of ISC markers (Lgr5, Olfm4, and Bmi1) and the Wnt/β-catenin pathway (Axin2) significantly increased in the primed MSC group. c The mRNA expressions of Muc2 (goblet cell), Alpi (enterocyte), Chga (enteroendocrine cell), and Occludin (epithelial tight junction) significantly increased in the primed MSC group. d IHC images of colon sections for lysozyme are shown. The lysozyme-expressing Paneth cells in crypt are counted from the normal mice, the DSS control, the unstimulated MSC-treated, and the primed MSC-treated mice (*p < 0.05, **p < 0.01, ***p < 0.001)

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Stem Cell Research & Therapy

ISSN: 1757-6512