Fig. 4

Bleomycin promotes annulus fibrosus cells’ migration in vitro. a, c AF cells were pretreated with TGFβR1 inhibitor LY364947(10 μM) or DMSO and stimulated with bleomycin in a concentration of 0, 5, and 10 μg/ml, then cells were cultured for 48 h and observed in a checkpoint of 0, 24 h and 48 h to calculate the migration rate by wound healing assay or cultured for 24 h to do the transwell test. b, d Normal and TGFβR1 knocked-down AF cells were stimulated with bleomycin in a concentration of 0, 5, and 10 μg/ml, then cells were cultured for 48 h and observed in a checkpoint of 0, 24, and 48 h to calculate the migration rate by wound healing assay or cultured for 24 h to do the transwell test