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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Effect of sex-specific differences on function of induced hepatocyte-like cells generated from male and female mouse embryonic fibroblasts

Fig. 2

Activation of external and internal transcriptional factors in induced miHeps. (A) Direct differentiation strategy for miHep generation. (B) Morphological changes during miHep generation, (a-1) male MEF and (b-1) female MEF upon initial seeding. After 1 month of induction, (a-2) male and (b-2) female MEFs successfully differentiated into male and female miHeps, respectively. The number of colonies increased with passage of time, and (a-3; b-3) both miHeps showed bipolar cells and polygonal morphology with various sizes of colonies. (C) The expression of (Ca) exogenic and (Cb) endogenic transcription factor genes in induced male and female miHeps. Male liver tissue were used as a negative control (Ca) or positive control (Cb) referenced to transcription factors. *Above the bars represent a statistically significant difference at p < 0.05. The data represent the relative quantification (RQ) ± min and max RQ values

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Stem Cell Research & Therapy

ISSN: 1757-6512