Skip to main content

Table 1 Sequences of primers used in qPCR

From: MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress

Gene

Primer

Sequence 5′–3′

Amplicon length (bp)

Accession no.

PPARγ

F:

TCCCTGTTTGTGTACAGCCTT

191

XM_014846252.1

R:

CTCCATGGCTGATTTCCCCT

ADIPOQ

F:

GGAGATCCAGGTCTTGTTGG

162

XM_014843352.1

R:

TCGGGTCTCCAATCCTACAC

Lep

F:

CACACGCAGTCAGTCTCCTC

176

XM_014854289.1

R:

CGGAGGTTCTCCAGGTCAT

CEBPA

F:

TCCCGGAGGGACCAAAGTTA

116

XM_023649498.1

R:

CTCACATTGCACAAGGCACC

AKT1

F:

CCAGGCTTGTGGTTGTCATCCT

108

XM_023628568.1

R:

TTCTTGAGGAGGAAGTACCGGG

AKT2

F:

CAGGAAACACAGGGAGCGG

160

XM_023649744.1

R:

GACACGCTGTCACCTAGCTC

SHBG

F:

GGCAACCTTTAACGCTCCAC

274

XM_023653464.1

R:

GACAGGCTTTTGTCCTGGGT

AHSG

F:

TGATGACCCCGAAACAGAGC

80

XM_005601872.3

R:

CGTGCTTGTAGCCCTGATGA

p53

F:

TACTCCCCTGCCCTCAACAA

252

U37120.1

R:

AGGAATCAGGGCCTTGAGGA

Bax

F:

TTCCGACGGCAACTTCAACT

204

XM_005596728.1

R:

GGTGACCCAAAGTCGGAGAG

Bcl-2

F:

TTCTTTGAGTTCGGTGGGGT

164

XM_014843802.1

R:

GGGCCGTACAGTTCCACAA

p21

F:

GAAGAGAAACCCCCAGCTCC

241

XM_003365840.2

R:

TGACTGCATCAAACCCCACA

Casp-3

F:

GGCAGACTTCCTGTATGCGT

167

XM_023630401.1

R:

CCATGGCTACCTTGCGGTTA

Casp-9

F:

TCCTACTCCACCTTCCCAGG

150

XM_005607504.3

R:

CTCCGAAACAGCGTGAGCTA

PERK

F:

GTGACTGCAATGGACCAGGA

283

XM_014852775.1

R:

TCACGTGCTCACGAGGATATT

CHOP

F:

AGCCAAAATCAGAGCCGGAA

272

XM_014844003.1

R:

GGGGTCAAGAGTGGTGAAGG

IRE1

F:

GAATCAGACGAGCACCCGAA

300

XM_023652216.1

R:

TTTCTTGCAGAGGCCGAAGT

ATF6

F:

CAGGGTGCACTAGAACAGGG

161

XM_023640315.1

R:

AATGTGTCTCCCCTTCTGCG

XBP1

F:

CGATCGAGTACTGTTGCCCT

299

XM_014742035.2

R:

GACGTTTGTCCAGTGACCCT

SOD1 (Cu/Zn SOD)

F:

CATTCCATCATTGGCCGCAC

130

NW_001867397.1

R:

GAGCGATCCCAATCACACCA

SOD2 (Mn SOD)

F:

GGACAAACCTGAGCCCCAAT

125

NW_001867408.1

R:

TTGGACACCAGCCGATACAG

CAT

F:

ACCAAGGTTTGGCCTCACAA

112

XM_014851065.1

R:

TTGGGTCAAAGGCCAACTGT

GAPDH

F:

GATGCCCCAATGTTTGTGA

250

NM_001163856.1

R:

AAGCAGGGATGATGTTCTGG

  1. Sequences: amplicon length and access numbers of the primer sets. PPARγ peroxisome proliferator-activated receptor gamma, ADIPOQ adiponectin, Lep leptin, CEBPA CCAAT/enhancer-binding protein alpha, AKT1 serine-threonine protein kinase 1, AKT2 serine-threonine protein kinase 2, SHBG sex hormone binding globulin, AHSG alpha 2-HS glycoprotein, p53 tumor suppressor p53, Bcl-2 B cell lymphoma 2, Bax BCl-2 associated X protein, p21 cyclin-dependent kinase inhibitor 1, Casp-3 caspase 3, Casp-9 caspase 9, PERK PRKR-like endoplasmic reticulum kinase, CHOP C/EBP homologous protein, ATF6 activating transcription factor 6, IRE1 inositol-requiring enzyme, XBP1 X-box binding protein 1, SOD1 (Cu/Zn SOD) copper-zinc-dependant superoxide dismutase (CuZnSOD), SOD2 (Mn SOD) manganese-dependent superoxide dismutase (MnSOD), CAT catalase, GADPH glyceraldehyde 3-phosphate dehydrogenase
Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512