Fig. 1
Morphology, proliferation, and phenotype of EpSlKCs and HHFKCs. a Representative light microscopy images of human epidermal stem-like keratinocyte (EpSlKC) and human hair follicular keratinocyte (HHFKC) culture. b DNA quantification of the cells along the culture time (n = 5, EpSlKCs; n = 4, HHFKCs). c Representative flow cytometry histograms and respective quantification regarding the percentage of α6bri/CD71dim cells, and those positive for integrin β1 (CD29), keratin 19 (K19), and keratin 14 (K14) in EpSlKC (n = 4) and HHFKC (n = 3) cultures after 1 week. d Immunofluorescence staining of β-actin filaments (phalloidin), keratin 14 (K14), keratin 10 (K10), keratin 6 (K6), keratin 15 (K15), and the proliferation-associated marker Ki67 in EpSlKCs and HHFKCs. DAPI was used as a nuclear counterstainer. Data shown are mean ± SEM. Scale bars are 100 μm for a and 50 μm for d. ***p < 0.001; ··p < 0.01 and ····p < 0.0001 vs. day 3; ###p < 0.001 and ####p < 0.0001 vs. day 5