Fig. 4

MiR-125a in HBMSCs was regulated by lncRNA-ANRIL and promoted the osteogenic differentiation of HBMSCs. a The binding sequence between miR-125a and ANRIL predicted using biological software. b Luciferase reporter assay was used to verify the correlation between ANRIL and miR-125a in 293T cells. c MiR-125a expression was measured by RT-PCR in the NC, ANRIL, shNC, and shANRIL groups. NC: HAMSCs + LPS + HBMSCs^NC; ANRIL: HAMSCs + LPS + HBMSCs^ANRIL; shNC: HAMSCs + LPS + HBMSCs^shNC; shANRIL: HAMSCs + LPS + HBMSCs^shANRIL. d Transfection efficacy of miR-125a was detected by RT-PCR. e ALP staining and activity in the NC, mimics, iNC, and inhibitor groups. Scale bar, 100 μm. f Alizarin red staining and quantification in the NC, mimics, iNC, and inhibitor groups. Scale bar, 100 μm. g Relative mRNA expression levels were measured by RT-PCR analysis. h Relative protein levels were assessed by Western blot analysis. Data are represented as mean ± standard error of the mean for both groups. ^*P < 0.05 and ^**P < 0.01. NC: HAMSCs + LPS + HBMSCs^{miR-125a NC}; mimics: HAMSCs + LPS + HBMSCs^{miR-125a mimics}; iNC: HAMSCs + LPS + HBMSCs^{miR-125a iNC}; inhibitor: HAMSCs + LPS + HBMSCs^{miR-125a inhibitor}