Fig. 1

Effect of GDF-5 on mouse EpSCs proliferation in vitro. a Flow cytometry was used to analyze the passaged EpSCs (P2). After the cells were passaged twice, the cells were labeled with CD49f and CD71 antibodies. EpSCs expressed high levels of CD49f and low levels of CD71. b Mouse primary EpSCs were treated with 0, 1, 50, 100, 500, and 1000 ng/ml of GDF-5 for 12, 24, 48, and 72 h. Cell proliferation was measured by CCK8. c EpSCs were treated by GDF-5 for 24 h, and the PCNA levels were analyzed by WB. The data were shown as the means ± SD of three independent experiments. ^aP < 0.01 vs. control (0 ng/ml GDF-5 as control), ^bP < 0.05 vs. control