Table 1 Primers for the RT-qPCR
From: GDF-5 promotes epidermal stem cells proliferation via Foxg1-cyclin D1 signaling
Primer name | Sequence (5′ to 3′) | Length | Tm (°C) |
|---|---|---|---|
GAPDH-F | GGTTGTCTCCTGCGACTTCA | 20 | 57.5 |
GAPDH-R | TGGTCCAGGGTTTCTTACTCC | 21 | 56.5 |
Foxa1-F | TTACAAGGATGCCTCTCCA | 19 | 52.6 |
Foxa1-R | TGGCTCTCTGAAAAGCAAG | 19 | 52.4 |
Foxc1-F | GGATCGGCTTGAACAACT | 18 | 52.4 |
Foxc1-R | AGAGTGCCGGGAATAGG | 17 | 54.0 |
Foxd3-F | CGTAGAGAAGCGTCGAGGA | 19 | 56.6 |
Foxd3-R | GGCAAAGGAGGTGTGAGTG | 19 | 56.5 |
Foxg1-F | AACGGGCTGAGTGTGGA | 17 | 58.8 |
Foxg1-R | CAGGGGTTGAGGGAGTAGG | 19 | 57.6 |
Foxo3-F | GAGGATTCGGCCATGCT | 17 | 55.4 |
Foxo3-R | TTCCTTGGTTGCCCAGAG | 18 | 55.1 |
Foxp1-F | TGCGCTGGACGATAGAA | 17 | 53.4 |
Foxp1-R | ATGCAGGTGGGTCATCA | 17 | 53.5 |
Cyclin C-F | ATGCTTGGTAATTGATTTGCT | 21 | 49.5 |
Cyclin C-R | CAGGGGTTGAGGGAGTAGG | 19 | 57.6 |
Cyclin D1-F | ACCCTGACACCAATCTCCT | 19 | 55.6 |
Cyclin D1-R | CTCCTTCTGCACGCACTT | 18 | 55.6 |
Cyclin D2-F | CCGTTCTTGGCTCTGGT | 17 | 55.1 |
Cyclin D2-R | AGGCACCTGTTGAAACTGA | 19 | 53.9 |
Cyclin D3-F | AAACCACGCCCCTGACT | 17 | 57.2 |
Cyclin D3-R | AGGTCCCACTTGAGCTTCC | 19 | 57.4 |
Cyclin E-F | CCCAAGTCCTGAGCCAT | 17 | 54.7 |
Cyclin E-R | TCGGAGCCACCTTCTTC | 17 | 54.5 |