Fig. 6

a Growth kinetics and b representative brightfield microscope images of hiPSCs seeded as single cells cultured in 0.1-L vertical-wheel bioreactors and serially passaged into 0.1-L and 0.5-L vertical-wheel bioreactors (scale bar = 200 μm). c Karyogram analysis of hiPSCs taken from the final day of the vertical-wheel bioreactor serial passage (day 12). Cell samples were collected from n = 4 stirred suspension bioreactors at each condition. The P values were set at 0.05 and all graphs are presented with a ± standard error of the mean (SEM)