Fig. 2
From: Cholangiogenic potential of human deciduous pulp stem cell-converted hepatocyte-like cells
SHED-HepT rescues biliary excretion in recipient CCl4-treated mice. a, b Levels of serum total bilirubin (a) and fecal bile acid (b) 4 weeks after transplantation by colorimetry. c Cholyl-L-lysyl-fluorescein (CLF) was intravenously infused into mice. The graphs showed the amount of CLF in serum, urine, and liver 2 h after the infusion. d–h Liver tissues were analyzed 4 weeks after SHED-HepT. Representative images of the livers were obtained by hematoxylin and eosin (H&E) staining (d) and by immunohistochemical analysis using human-specific antibodies to membrane metalloendopeptidase (MME; e, f), keratin 19 (KRT19; g), and KRT7 (KRT7; h). Nuclei were stained with hematoxylin. a–c n = 5 for all groups. **P < 0.01, ***P < 0.005. nd, no detection; ns, no significance. The graph bars represented the means ± SEM. d arrow: bile duct. d–h Scale bars, 20 μm (d, f-h) and 50 μm (e). d, f–h PV, portal vein