Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis

Fig. 1

Generation of neurosphere from hiPS/ESCs. a The protocol for deriving neurospheres from H9 and UiPS. b The morphology and Immunostaining of H9 and UiPS at days − 1 (a, b, f, g), day 5 (c, h), and day 7 (d, e, i, j) during neural induction. Immunostaining showing emergence of SSEA4+ (green), OCT4+ (red) H9 and UiPS (b, g), and SOX2+ (red), NESTIN+ (green) neurospheres induced from H9 and UiPS at day 7 (e, j). Scale bars = 100 μm. Nuclei were counterstained with DAPI (blue). c Quantitative RT-PCR analysis of the expression level of neural progenitor markers SOX1, PAX6, NESTIN, and SOX2 and pluripotency markers NANOG and OCT4 of H9 and H9-neurospheres. d Quantitative RT-PCR analysis of the expression level of neural progenitor markers SOX1, PAX6, NESTIN, and SOX2 and pluripotency markers NANOG and OCT4 of UiPS and UiPS-neurospheres. n = 3 each. Data are expressed as means ± SD.*P < 0.05, **P < 0.01 vs. control

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512