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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis

Fig. 2

lt-NES cells can be generated from H9/UiPS-neurospheres under a chemically defined culture system using N2+AA medium. a The lt-NES cells derivation protocol from neurospheres. b The morphologies of the lt-NES cells in N2 medium (coated/uncoated) and N2+AA (AA). Scale bars = 100 μm. c The viability of P0 lt-NES cells was assessed by a CCK-8 assay after plated for 12 h. d Expansion capacity of lt-NES cells investigated by continuous culture in N2 medium (coated/uncoated) and N2+AA (AA) in 8 days. n = 3 each

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Stem Cell Research & Therapy

ISSN: 1757-6512