Fig. 7

Effect of hFDSPC-CM on cell migration of HaCat and human fibroblasts was tested using the scratch assay in vitro. The scratch was made when cultured cells reached a confluent monolayer. Then, cells were treated with PBS/hFDSPC-CM/hADSC-CM at a concentration of 20 μg/mL and cultured for 24 h. The data acquired from four randomly selected fields were quantified as a percentage of the area of the scratch filled with cells. HaCaT migration was observed at 0 and 24 h (a), and the migrated area was measured (b). Human fibroblast migration was evaluated (c), and the migrated area was quantified (d). Data are shown as means ± SD; bar = 100 μm; n = 4; *p < 0.05, ***p < 0.001