Table 1 Primer sequences used for real-time qPCR
Gene | Species | Forward primer | Reverse primer |
|---|---|---|---|
ALP | Human | TACAAGCACTCCCACTTCATC | AGACCCAATAGGTAGTCCACAT |
BMP-2 | Human | GAAGAACTACCAGAAACGAGTG | GGTGATGGAAACTGCTATTG |
Osx | Human | CAGTTGATAGGGTTTCTCTTGTA | CATAGGACTTGAGGTTTCACAG |
OCN | Human | CTGTGACGAGTTGGCTGAC | AGCAGAGCGACACCCTAGA |
OPN | Human | CATTCCGATGTGATTGATAGTC | CTTCCTTACTTTTGGGGTCTAC |
C/EBP α | Human | CCCTCAGCCTTGTTTGTACT | AAAATGGTGGTTTAGCAGAGA |
FABP4 | Human | AGAGAAAACGAGAGGATGATAA | TTCAATGCGAACTTCAGTC |
PPAR-γ2 | Human | GCAGTGGGGATGTCTCATAAT | CAGCGGACTCTGGATTCAG |
SREBP1 | Human | AACACAGCAACCAGAAACTCA | GTCCTCCACCTCAGTCTTCAC |
COL 2 | Human | TGGACGCCATGAAGGTTTTCT | TGGGAGCCAGATTGTCATCTC |
Aggrecan | Human | GTGCCTATCAGGACAAGGTCT | GATGCCTTTCACCACGACTTC |
SOX-9 | Human | AGCGAACGCACATCAAGAC | CTGTAGGCGATCTGTTGGGG |
COL 1 | Human | AGGGCCAAGACGAAGACATC | GTCGGTGGGTGACTCTGAGC |
COL3 | Human | AAGGGCAGGGAACAACT | ATGAAGCAGAGCGAGAAG |
MMP-1 | Human | GGAGCTGTAGATGTCCTTGGGGT | GCCACAACTGCCAAATGGGCTT |
MMP-3 | Human | AGGACAAAGCAGGATCACAGTTG | CCTGGTACCCACGGAACCT |
FN | Human | TCTCCTGCCTGGTACAGAATAT | GGTCGCAGCAACAACTTCCAGGT |
α-SMA | Human | GCTACTCCTTCGTGACCACAG | GCCGTCGCCATCTCGTTCT |
TGF-β1 | Human | TACTACGCCAAGGAGGTCAC | GAGAGCAACACGGGTTCAG |
β-actin | Human | GGCACTCTTCCAGCCTTCC | GAGCCGCCGATCCACAC |