Fig. 6

GDF7 impairs the suppressive effect of miR-204-5p on the expression of RUNX2, ALP, OCN, p-AKT and p-P38. hADSCs were co-transfected with miR-204-5p mimics and GDF7-overexpression vector or empty vector or co-transfected with miR-204-5p inhibitors and si-GDF and cultured in OM. a Relative expression of miR-204-5p, GDF7, RUNX2, ALP and OCN measured by qRT-PCR on day 14 of osteogenic induction. U6 and GAPDH were used as internal control genes. b Left panel: Western blot of GDF7, RUNX2, ALP and OCN protein levels on day 14 after osteogenic induction. GAPDH was used as an internal control. Right panel: quantitative analysis of the protein levels. c Left panel: Western blot of p-AKT, AKT, p-P38 and P38 protein levels on day 14 after osteogenic induction. GAPDH was used as an internal control. Right panel: quantitative analysis of the protein levels. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 independent experiments)