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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Expression and function of Ndel1 during the differentiation of neural stem cells induced by hippocampal exosomesticle

Fig. 4

Effects of Ndel1 on NSC differentiation. a RT-qPCR analysis of Map2 and Neurod1 expression. (LV-NC) NSCs treated with negative control of overexpression lentivirus; (LV-Ndel1) NSCs treated with overexpression lentivirus of Ndel1; (LV-NCi) NSCs treated with negative control of interference lentivirus; (LV-Ndel1i) NSCs treated with interference lentivirus of Ndel1. b Western blot analysis of Tuj1 and MAP2 protein levels. c, d The percentage of Tuj1-positive cells detected by flow cytometry. e, f Cell differentiation was detected by immunofluorescence. Nuclei were stained with Hoechst. Scale bar = 200 μm. Values are mean ± SEM from three biological replicates; *P < 0.05, **P < 0.01, ***P < 0.001

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