Fig. 5

The miR-107-3p regulated NSC differentiation by targeting Ndel1. a Analysis of miR-107-3p expression in different tissues. b, c RT-qPCR and Western blot analysis confirming the changes in Ndel1 expression. (miR-NC) NSCs treated with negative control of miRNA mimic; (miR-107-3p) NSCs treated with miR-107-3p mimic; (miR-NCi) NSCs treated with negative control of miRNA inhibitor; (miR-107-3pi) NSCs treated with miR-107-3p inhibitor. d Luciferase reporter assay in HEK-293 cells co-transfected with Luciferase-miR-107-3p fusion and Ndel1-Wild or Ndel1-Mut, 72 h post-transfection. e RT-qPCR analysis of Map2 and Neurod1 expression. (miR-NC) NSCs treated with negative control of miRNA mimic; (miR-107-3p) NSCs treated with miR-107-3p mimic; (miR-107-3p+LV-Ndel1) NSCs treated with miR-107-3p mimic and overexpression lentivirus of Ndel1. f Western blot analysis of Tuj1 and MAP2 protein levels. g, h The percentage of Tuj1-positive cells detected by flow cytometry. i, j Cell differentiation was detected by immunofluorescence. Nuclei were stained with Hoechst. Scale bar = 200 μm. Values are mean ± SEM from three biological replicates; *P < 0.05, **P < 0.01, ***P < 0.001