Fig. 3

Inhibition of miR-126 downregulates protein expression of Akt/eNOS and ERK/VEGF signaling pathways. Western blotting shows immunochemistry signals of phosphorylated-ERK (p-ERK), ERK, VEGF, p-Akt, Akt, and eNOS in LOCs transfected with negative control or miR-126 inhibitor (a). Quantification of p-ERK (b), VEGF (c), p-Akt (d), and eNOS (e) bands. GADPH expression was used for protein level normalization for VEGF and eNOS. Data are presented as mean ± SD. **P < 0.01, ***P < 0.001 vs. respective control group; n ≥ 3