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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway

Fig. 1

Exosomes derived from MSCs pretreated with MIF protected cardiomyocytes from apoptosis. Confirmation of exosomal collection by TEM, NTA, and western blot. a Representative TEM image. b Size range of plasma exosomes determined by NTA analysis. c Representative western blot images showing exosomal markers and β-actin. MSCs were treated with or without MIF. The exosomes were then collected and added to cardiomyocytes treated with H2O2. Cardiomyocytes without treatment were used as a control. d, e Representative flow cytometric dot plots of apoptotic cells after Annexin V/propidium iodide staining. Activities of f caspases 3/7 and g caspase 8 in cell lysates measured by ELISA. Each column represents mean ± SD of three independent experiments. *P < 0.05 vs. control; P < 0.05 vs. H2O2+exosomeMIF

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