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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Exosomes from bone marrow mesenchymal stem cells enhance fracture healing through the promotion of osteogenesis and angiogenesis in a rat model of nonunion

Fig. 1

Characterization of BMMSCs and BMMSC-Exos. a Fusiform morphology of BMMSCs shown in light microscopy images. b Alizarin red staining was performed to detect the osteogenic differentiation ability of BMMSCs: B1, staining of experimental group; B2, staining of control group; B3, gross scanning images of ARS staining of experimental group. c Oil red staining was performed to detect the lipid differentiation ability of BMMSCs: C1, staining of the experimental group; C2, staining of the control group. d Surface markers of BMMSCs analysed by flow cytometry. The cells were negative for CD34 and CD11b/C and positive for CD90 and CD29. e The morphology of BMMSC-Exos shown by TEM. f Image of the purified exosomes. g The particle size distribution in purified BMMSC-Exos determined by NTA. h The surface markers (CD9, CD63 and CD81) of exosomes were detected by western blotting

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Stem Cell Research & Therapy

ISSN: 1757-6512